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The critically acclaimed laboratory standard for almost 50 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Each volume is eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 520 volumes and 40,000 chapters in the collection, much of the material is still relevant today and is truly an essential publication for researchers in all fields of life sciences, including microbiology, biochemistry, cancer research, and genetics, just to name a few. In this volume, number 545, we have brought

Enzymes -- Analysis. --- Proteins -- Analysis. --- Proteins -- Purification. --- Proteins --- Enzymes --- Analysis. --- Purification. --- Proteids --- Biomolecules --- Polypeptides --- Proteomics

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Enzymology --- 577.15 --- Enzymes. Catalysts of biological reactions. Enzymology --- 577.15 Enzymes. Catalysts of biological reactions. Enzymology --- Subject headings --- Enzymes --- Biochemistry --- Analysis --- Technique --- Enzymes - Analysis - Indexes --- Biochemistry - Technique - Indexes --- ENZYMES --- INDEXES

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General biochemistry --- enzymen --- radiometrische analyse --- biotechnologie --- biochemie --- polarografie --- Enzymology --- statistisch onderzoek --- HPLC (high power liquid chromatography) --- O (zuurstof) --- polyacrylamidegelelektroforese --- fotometrie --- buffers (chemie) --- pH-metrie --- Biotechnology --- Enzymes --- Analysis --- Purification --- Analysis. --- Purification. --- chromatografie --- LC (liquid chromatography) --- Enzymes - Analysis --- Enzymes - Purification

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Enzymatic Analysis: A Practical Guide is a multipurpose manual of laboratory methods. It offers a systematic scheme for the analysis of biological materials from the level of the whole organ down to the single cell and beyond. It is intended as a guide to the development of new methods, to the refinement of old ones, and to the adaptation in general of methods to almost any scale of sensitivity. As some may realize, the book is a sequel to A Flexible System of Enzymatic Analysis, originally published in 1972. The major changes, other than an appropriate interchange of authors, consist of a wholly new chapter of methods and protocols for measuring enzymes, the addition of 13 new entries in the metabolite chapter, and a much superior chapter on enzymatic cycling. With considerable nostalgia, we have switched from DPN and TPN to NAD and NADP nomenclature, which no doubt will make Otto Warburg turn over in his grave. The incentives for the methodology in this book came from the rigorous demands of quantitative histochemistry and cytochemistry. These demands are specificity, simplicity, flexibility, and, of course, sensitivity—all likewise desirable attributes of methods for other purposes. The specificity is provided by the use of enzyme methods. Simplicity is achieved by leading all reactions to a final pyridine nucleotide step.

Enzymes --- Histocytochemistry --- Analysis --- Laboratory manuals --- analysis --- laboratory manuals --- Chemistry. --- Enzymes -- Analysis -- Laboratory manuals. --- Enzymes. --- Biochemistry --- Histological Techniques --- Histology --- Cytological Techniques --- Enzymes and Coenzymes --- Chemistry --- Anatomy --- Biological Science Disciplines --- Clinical Laboratory Techniques --- Chemicals and Drugs --- Investigative Techniques --- Natural Science Disciplines --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Disciplines and Occupations --- Animal Biochemistry --- Human Anatomy & Physiology --- Health & Biological Sciences --- Life sciences. --- Biochemistry. --- Life Sciences. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Medical sciences --- Biosciences --- Sciences, Life --- Science --- Composition --- Biocatalysts --- Ferments --- Soluble ferments --- Catalysts --- Proteins --- Enzymology --- laboratory manuals. --- Enzymes - Analysis - Laboratory manuals

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The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.

577.15 --- 577.15 Enzymes. Catalysts of biological reactions. Enzymology --- Enzymes. Catalysts of biological reactions. Enzymology --- Enzymologie --- Enzymologie. --- Carbohydrates --- Enzymes --- Biocatalysts --- Ferments --- Soluble ferments --- Catalysts --- Proteins --- Enzymology --- Carbs (Carbohydrates) --- Biomolecules --- Organic compounds --- Glycomics --- Biological techniques --- Biochemistry --- Carbohydrates. --- Enzymen. --- Enzymes. --- Technique. --- Technique --- Addresses, essays, lectures --- Complex compounds --- Analysis --- Enzymes - Analysis --- GLYCOCONJUGATE --- ANTIBODIES --- ANALYSIS --- ISOLATION AND PURIFICATION --- METABOLISM --- BIOSYNTHESIS